Collagen X Recombinant Rabbit Monoclonal Antibody [JF0961]
cat.: ET1702-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat, Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF0961 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Collagen X aa 550-599 / 680. |
| Positive control: | Rat liver tissue lysates, human liver tissue lysates. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q03692 Human | Q05306 Mouse Unigene: 20579 Rat |
| Alternative names: | COAA1_HUMAN Col10a 1 COL10A1 Collagen alpha 1(X) chain Collagen alpha-1(X) chain Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) Collagen type X alpha 1 Collagen X alpha 1 polypeptide CollagenX fa66d11 fb10c08 OTTHUMP00000040411 Procollagen type X alpha 1 Schmid metaphyseal chondrodysplasia wu:fa66d11 wu:fb10c08 |
Images
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Fig1:
Western blot analysis of Collagen X on rat liver tissue lysates with Rabbit anti-Collagen X antibody (ET1702-13) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Collagen X on human liver tissue lysates with Rabbit anti-Collagen X antibody (ET1702-13) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-13) at 1/1,000dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.