FOXP3 Recombinant Rabbit Monoclonal Antibody [JF0926]
cat.: ET1702-16
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0926 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FOXP3 aa 282-431 / 431. |
| Positive control: | Raji, human lymph node tissue, human tonsil tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB FC IHC-P |
1:500-1:1,000 1:10-1:50 1:200 |
| Uniprot #: | SwissProt: Q9BZS1 Human |
| Alternative names: | AIID DIETER Forkhead box P3 Forkhead box protein P3 FOXP3 FOXP3_HUMAN FOXP3delta7 Immune dysregulation polyendocrinopathy enteropathy X linked Immunodeficiency polyendocrinopathy enteropathy X linked IPEX JM2 MGC141961 MGC141963 OTTHUMP00000025832 OTTHUMP00000025833 OTTHUMP00000226737 PIDX Scurfin XPID |
Images
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Fig1: Flow cytometric analysis of Raji cells with FOXP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-FOXP3 antibody (ET1702-16) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-FOXP3 antibody (ET1702-16) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.