FOXP3 Recombinant Rabbit Monoclonal Antibody [JF0926]
cat.: ET1702-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC, IHC-P
Clonality: Monoclonal
Clone number: JF0926
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FOXP3 aa 282-431 / 431.
Positive control: Raji, human lymph node tissue, human tonsil tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  FC
  IHC-P

1:500-1:1,000
1:10-1:50
1:200
Uniprot #: SwissProt: Q9BZS1 Human
Alternative names: AIID DIETER Forkhead box P3 Forkhead box protein P3 FOXP3 FOXP3_HUMAN FOXP3delta7 Immune dysregulation polyendocrinopathy enteropathy X linked Immunodeficiency polyendocrinopathy enteropathy X linked IPEX JM2 MGC141961 MGC141963 OTTHUMP00000025832 OTTHUMP00000025833 OTTHUMP00000226737 PIDX Scurfin XPID
Images
ET1702-16_1.jpg Fig1: Flow cytometric analysis of Raji cells with FOXP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ET1702-16_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-FOXP3 antibody (ET1702-16) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-16_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-FOXP3 antibody (ET1702-16) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.