STAT2 Recombinant Rabbit Monoclonal Antibody [JF0884]
cat.: ET1702-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JF0884
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 98 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human STAT2 aa 1-49/851.
Positive control: A431 cell lysate, THP-1 cell lysate, Ramos cell lysate, Hela cell lysate, K562 cell lysate, A431, A549, SW480, human lung cancer tissue, mouse skin cancer tissue, human colon cancer tissue, rat colon tissue, rat kidney tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:200
1:100
Uniprot #: SwissProt: P52630 Human | Q9WVL2 Mouse
Unigene: 24237 Rat
Alternative names: Homo sapiens interferon alpha induced transcriptional activator interferon alpha induced transcriptional activator ISGF 3 ISGF3 MGC59816 P113 signal transducer and activator of transcription 2 113kD Signal transducer and activator of transcription 2 STAT113 Stat2 STAT2_HUMAN
Images
ET1702-18_1.jpg Fig1: Western blot analysis of STAT2 on different lysates with Rabbit anti-STAT2 antibody (ET1702-18) at 1/1,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (40 µg/Lane)

Predicted band size: 98 kDa
Observed band size: 105 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-18) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature."
ET1702-18_2.jpg Fig2: Western blot analysis of STAT2 on different lysates with Rabbit anti-STAT2 antibody (ET1702-18) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: K562 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 98 kDa
Observed band size: 105 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1702-18_3.jpg Fig3: Immunocytochemistry analysis of A431 cells labeling STAT2 with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (ET1702-18) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1702-18_4.jpg Fig4: Immunocytochemistry analysis of A549 cells labeling STAT2 with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (ET1702-18) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1702-18_5.jpg Fig5: Immunocytochemistry analysis of SW480 cells labeling STAT2 with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-STAT2 antibody (ET1702-18) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1702-18_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-18) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-18_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin cancer tissue with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-18) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-18_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-18) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-18_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-18) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-18_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-STAT2 antibody (ET1702-18) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-18) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-18_11.jpg Fig11: Flow cytometric analysis of A431 cells labeling STAT2.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-18, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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