Tissue type plasminogen activator Recombinant Rabbit Monoclonal Antibody [JF0958]
cat.: ET1702-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0958 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Tissue-type plasminogen activator aa 523-562 / 562. |
| Positive control: | Rat brain tissue, mouse brain tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P00750 Human | P11214 Mouse | P19637 Rat |
| Alternative names: | Alteplase DKFZp686I03148 Plasminogen activator tissue Plasminogen activator tissue type PLAT Reteplase t PA T Plasminogen Activator t-PA T-plasminogen activator Tissue plasminogen activator (t PA) Tissue type plasminogen activator Tissue-type plasminogen activator chain B tPA TPA_HUMAN TPA1 |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tissue type plasminogen activator antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tissue type plasminogen activator antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.