Acetyl CoA synthetase Recombinant Rabbit Monoclonal Antibody [JF0917]
cat.: ET1702-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JF0917 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Acetyl CoA synthetase aa 101-145 / 701. |
| Positive control: | HepG2 cell lysate, L6 cell lysate, COS-1 cell lysate, RH-35, SW480. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q9NR19 Human | Q8BK97 Mouse | Q9QXG4 Mouse | D3ZFF9 Rat | D3ZUL6 Rat |
| Alternative names: | ACAS2 AceCS Acetate CoA ligase Acetate thiokinase Acetate--CoA ligase Acetyl CoA synthetase Acetyl Coenzyme A synthetase 2 (ADP forming) Acetyl coenzyme A synthetase cytoplasmic Acetyl-CoA synthetase Acetyl-coenzyme A synthetase ACS ACSA ACSA_HUMAN ACSS2 Acyl activating enzyme Acyl CoA synthetase short chain family member 2 Acyl-activating enzyme Acyl-CoA synthetase short-chain family member 2 Cytoplasmic acetyl coenzyme A synthetase cytoplasmic MYH7B |
Images
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Fig1:
Western blot analysis of Acetyl CoA synthetase on different lysates with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: L6 cell lysate Lane 3: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Acetyl CoA synthetase on different lysates with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Acetyl CoA synthetase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of Acetyl CoA synthetase in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Acetyl CoA synthetase in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Immunocytochemistry analysis of HEPG2 cells labeling Acetyl CoA synthetase with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
"Immunocytochemistry analysis of L6 cells labeling Acetyl CoA synthetase with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution." |
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