Telomerase reverse transcriptase Recombinant Rabbit Monoclonal Antibody [JF0586]
cat.: ET1702-22
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: JF0586
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 127/120/90/89 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Telomerase reverse transcriptase aa 1090-1132/1132.
Positive control: HeLa cell lysates, HeLa.
Subcellular location: Nucleus, Chromosome, Cytoplasm.
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: O14746 Human
Alternative names: CMM9 DKCA2 DKCB4 EST2 HEST2 htert hTRT PFBMFT1 TCS1 Telomerase associated protein 2 Telomerase catalytic subunit Telomerase reverse transcriptase Telomerase-associated protein 2 Telomere Reverse Transcriptase TERT TERT_HUMAN TP2 TRT
Images
ET1702-22_1.jpg Fig1: Western blot analysis of Telomerase reverse transcriptase on HeLa cell lysates with Rabbit anti-Telomerase reverse transcriptase antibody (ET1702-22) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-22) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1702-22_2.jpg Fig2: Flow cytometric analysis of HeLa cells with TERT antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.