Telomerase reverse transcriptase Recombinant Rabbit Monoclonal Antibody [JF0586]
cat.: ET1702-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0586 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 127/120/90/89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Telomerase reverse transcriptase aa 1090-1132/1132. |
| Positive control: | HeLa cell lysates, HeLa. |
| Subcellular location: | Nucleus, Chromosome, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: O14746 Human |
| Alternative names: | CMM9 DKCA2 DKCB4 EST2 HEST2 htert hTRT PFBMFT1 TCS1 Telomerase associated protein 2 Telomerase catalytic subunit Telomerase reverse transcriptase Telomerase-associated protein 2 Telomere Reverse Transcriptase TERT TERT_HUMAN TP2 TRT |
Images
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Fig1:
Western blot analysis of Telomerase reverse transcriptase on HeLa cell lysates with Rabbit anti-Telomerase reverse transcriptase antibody (ET1702-22) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-22) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of HeLa cells with TERT antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.