Telomerase reverse transcriptase Recombinant Rabbit Monoclonal Antibody [JF0586]
cat.: ET1702-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0586 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Telomerase reverse transcriptase aa 1090-1132/1132. |
| Positive control: | MCF7 (Human breast cancer cell) cell lysate, C2C12 (Mouse myoblast) cell lysate. |
| Subcellular location: | Nucleus, Chromosome, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: O14746 Human | O70372 Mouse |
| Alternative names: | CMM9 DKCA2 DKCB4 EST2 HEST2 htert hTRT PFBMFT1 TCS1 Telomerase associated protein 2 Telomerase catalytic subunit Telomerase reverse transcriptase Telomerase-associated protein 2 Telomere Reverse Transcriptase TERT TERT_HUMAN TP2 TRT |
Images
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Fig1:
Western blot analysis of Telomerase reverse transcriptase on different lysates with Rabbit anti-Telomerase reverse transcriptase antibody (ET1702-22) at 1/500 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: C2C12 (Mouse myoblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-22, 1/500 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 127 kDa Observed band size: 127 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.