PDIA6 Recombinant Rabbit Monoclonal Antibody [JF0974]
cat.: ET1702-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0974 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within PDIA6 aa 1-108 / 440. |
| Positive control: | HepG2 cell lysate, K562 cell lysate, Hela, HepG2, NIH/3T3. |
| Subcellular location: | Endoplasmic reticulum lumen, Cell membrane, Melanosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC |
1:1,000-1:2,000 1:100-1:500 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q15084 Human | Q922R8 Mouse | Q63081 Rat |
| Alternative names: | Endoplasmic reticulum protein 5 ER protein 5 ERp5 P5 Pdia6 PDIA6_HUMAN Protein disulfide isomerase A6 Protein disulfide isomerase associated 6 Protein disulfide isomerase family A member 6 Protein disulfide isomerase P5 Protein disulfide isomerase related protein Protein disulfide-isomerase A6 Thioredoxin domain containing 7 (protein disulfide isomerase) Thioredoxin domain containing protein 7 Thioredoxin domain-containing protein 7 TXNDC7 |
Images
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Fig1:
Western blot analysis of PDIA6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysate |
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Fig2: ICC staining of PDIA6 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of PDIA6 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of PDIA6 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Flow cytometric analysis of PDIA6 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.