PDIA6 Recombinant Rabbit Monoclonal Antibody [JF0974]
cat.: ET1702-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0974 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within PDIA6 aa 1-108 / 440. |
| Positive control: | HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, 4T1 cell lysate, PC-12 cell lysate, Rat liver tissue lysate, PC-12, human liver tissue. |
| Subcellular location: | Endoplasmic reticulum lumen, Cell membrane, Melanosome. |
| Recommended Dilutions:
WB FC IF-Cell IHC-P |
1:1,000-1:2,000 1:1:1,000 1:100 1:5,000 |
| Uniprot #: | SwissProt: Q15084 Human | Q922R8 Mouse | Q63081 Rat |
| Alternative names: | Endoplasmic reticulum protein 5 ER protein 5 ERp5 P5 Pdia6 PDIA6_HUMAN Protein disulfide isomerase A6 Protein disulfide isomerase associated 6 Protein disulfide isomerase family A member 6 Protein disulfide isomerase P5 Protein disulfide isomerase related protein Protein disulfide-isomerase A6 Thioredoxin domain containing 7 (protein disulfide isomerase) Thioredoxin domain containing protein 7 Thioredoxin domain-containing protein 7 TXNDC7 |
Images
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Fig1:
Western blot analysis of PDIA6 on different lysates with Rabbit anti-PDIA6 antibody (ET1702-26) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: 4T1 cell lysate Lane 6: PC-12 cell lysate Lane 7: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: Lane 1-3: 25 seconds; Lane 4-7: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling PDIA6 with Rabbit anti-PDIA6 antibody (ET1702-26) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PDIA6 antibody (ET1702-26) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PDIA6 antibody (ET1702-26) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-26) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of PC-12 cells labeling PDIA6. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-26, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.