LYVE1 Recombinant Rabbit Monoclonal Antibody [JF0979]
cat.: ET1702-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0979 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LYVE1 aa 36-72 / 322. |
| Positive control: | MCF-7 cell lysate, SW480 cell lysate, A431 cell lysate, HUVEC cell lysate, A431, HUVEC, SW480. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y5Y7 Human |
| Alternative names: | Cell surface retention sequence-binding protein 1 CRSBP 1 CRSBP-1 CRSBP1 extracellular link domain containing 1 extracellular link domain-containing 1 Extracellular link domain-containing protein 1 HAR Hyaluronic acid receptor Lymphatic endothelium specific hyaluronan receptor lymphatic vessel endothelial hyaluronan receptor 1 Lymphatic vessel endothelial hyaluronic acid receptor 1 LYVE 1 LYVE-1 LYVE1 LYVE1_HUMAN XLKD1 |
Images
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Fig1:
Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (ET1702-29) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: SW480 cell lysate Lane 3: A431 cell lysate Lane 4: HUVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-29) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (ET1702-29) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si LYVE1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1702-29 was shown to specifically react with LYVE1 in A549-si NT cells. Weakened band was observed when A549-si LYVE1 sample was tested. A549-si NT and Hela-si LYVE1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: Flow cytometric analysis of LYVE1 was done on HUVEC cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-29, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.