Thymidine Kinase 1 Recombinant Rabbit Monoclonal Antibody [JF0970]
cat.: ET1702-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0970 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Thymidine Kinase 1 aa 2-49 / 234. |
| Positive control: | HeLa cell lysate, A549 cell lysate, human tonsil tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P04183 Human |
| Alternative names: | cytosolic KITH_HUMAN Thymidine kinase 1 Thymidine kinase 1 soluble Thymidine kinase 1 soluble isoform Thymidine kinase Thymidine kinase cytosolic TK 1 TK 2 TK1 Tk1a Tk1b TK2 |
Images
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Fig1:
Western blot analysis of Thymidine Kinase 1 on different lysates with Rabbit anti-Thymidine Kinase 1 antibody (ET1702-31) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Thymidine Kinase 1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Thymidine Kinase 1 on different lysates with Rabbit anti-Thymidine Kinase 1 antibody (ET1702-31) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 antibody (ET1702-31) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.