gamma Tubulin Recombinant Rabbit Monoclonal Antibody [JF0972]
cat.: ET1702-32
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JF0972
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Tubulin gamma-2 chain aa 227-407 / 451.
Positive control: HeLa cell lysate, A431 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HeLa, NIH/3T3, human testis tissue, mouse testis tissue, rat testis tissue, Jurkat.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:2,000
1:100
1:50-1:200
1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P23258 Human | Q9NRH3 Human | P83887 Mouse | Q8VCK3 Mouse | P83888 Rat | P23330 XenopusLaevis
Alternative names: Gamma 1 tubulin Gamma 2 tubulin Gamma Tubulin 1 Gamma Tubulin 2 Gamma tubulin complex component 1 Gamma-2-tubulin GCP 1 GCP1 MGC131994 TBG2_HUMAN TUBG TUBG1 TUBG2 TUBGCP1 Tubulin gamma 1 chain Tubulin gamma 2 chain Tubulin gamma complex-associated protein 1 Tubulin gamma-2 chain tubulin, gamma 1 tubulin, gamma 2 tubulin, gamma polypeptide Xgam
Images
ET1702-32_1.jpg Fig1: Western blot analysis of gamma Tubulin on different lysates with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: COS-1 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 48 kDa

Exposure time: 12 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-32) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1702-32_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling gamma Tubulin with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at RT, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1702-32_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling gamma Tubulin with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1702-32_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-32) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-32_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-32) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-32_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-gamma Tubulin antibody (ET1702-32) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-32) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-32_7.jpg Fig7: Flow cytometric analysis of gamma Tubulin was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-32, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.