ET1702-32

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality:	Monoclonal
Clone number:	JF0972

Product Type:

Recombinant Rabbit monoclonal IgG, primary antibodies

Species reactivity:

Human, Mouse, Rat, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis

Applications:

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Clonality:

Monoclonal

Clone number:

JF0972

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	51 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human Tubulin gamma-2 chain aa 227-407 / 451.
Positive control:	Jurkat cell lysate, A431 cell lysate, Hela, MCF-7, SHG-44, mouse colon tissue, mouse testis tissue, Jurkat.
Subcellular location:	Cytoplasm.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC IP	1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.
Uniprot #:	SwissProt: P23258 Human \| Q9NRH3 Human \| P83887 Mouse \| Q8VCK3 Mouse \| P83888 Rat \| P23330 XenopusLaevis
Alternative names:	Gamma 1 tubulin Gamma 2 tubulin Gamma Tubulin 1 Gamma Tubulin 2 Gamma tubulin complex component 1 Gamma-2-tubulin GCP 1 GCP1 MGC131994 TBG2_HUMAN TUBG TUBG1 TUBG2 TUBGCP1 Tubulin gamma 1 chain Tubulin gamma 2 chain Tubulin gamma complex-associated protein 1 Tubulin gamma-2 chain tubulin, gamma 1 tubulin, gamma 2 tubulin, gamma polypeptide Xgam

	Fig1: Western blot analysis of gamma Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-gamma Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Flow cytometric analysis of gamma Tubulin was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-32, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).