gamma Catenin Recombinant Rabbit Monoclonal Antibody [JF0973]
cat.: ET1702-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0973 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 82 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human gamma Catenin aa 10-116 / 745. |
| Positive control: | Hela cell lysate, human skin tissue lysate, Hela, MCF-7, A431, mouse stomach tissue. |
| Subcellular location: | Cytoskeleton, adherens junction, desmosome, Membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:2,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P14923 Human | Q02257 Mouse | Q6P0K8 Rat |
| Alternative names: | ARVD 12 ARVD12 Catenin (cadherin associated protein), gamma 80kDa catenin (cadherin-associated protein) gamma (80kD) Catenin gamma CTNNG Desmoplakin 3 Desmoplakin III Desmoplakin-3 Desmoplakin3 DesmoplakinIII DP 3 DP III DP3 DPIII gamma catenin Junction plakoglobin JUP OTTHUMP00000164732 OTTHUMP00000164735 OTTHUMP00000164738 PDGB PKGB PLAK_HUMAN PLAKOGLOBIN |
Images
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Fig1: ICC staining of gamma Catenin in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of gamma Catenin in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of gamma Catenin in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-gamma Catenin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of gamma Catenin was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-33, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Western blot analysis of gamma Catenin on different lysates with Rabbit anti-gamma Catenin antibody (ET1702-33) at 1/1,000 dilution. Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-33, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 82 kDa Observed band size: 82 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.