Cardiac Troponin I Recombinant Rabbit Monoclonal Antibody [JF0550]
cat.: ET1702-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JF0550 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cardiac Troponin I aa 1-47 / 210. |
| Positive control: | Human heart tissue, HepG2. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:2,000 1:50-1:200 1:200 1:1,000 |
| Uniprot #: | SwissProt: P19429 Human | P23693 Rat |
| Alternative names: | cardiac muscle Cardiac troponin I cardiomyopathy, dilated 2A (autosomal recessive) Cardiomyopathy, familial hypertrophic, 7, included CMD1FF CMD2A CMH7 cTnI Familial hypertrophic cardiomyopathy 7 MGC116817 RCM1 Tn1 Tni TNN I3 TNNC 1 TNNC1 TNNI3 TNNI3_HUMAN Troponin I Troponin I cardiac Troponin I cardiac muscle Troponin I cardiac muscle isoform Troponin I type 3 cardiac troponin I, cardiac 3 TroponinI Ttroponin I type 3 (cardiac) |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin I with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-37, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3: ICC staining of Cardiac Troponin I in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.