Cardiac Troponin I Recombinant Rabbit Monoclonal Antibody [JF0550]
cat.: ET1702-37
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: JF0550
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 24 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cardiac Troponin I aa 1-47 / 210.
Positive control: Rat heart tissue lysate, human heart tissue, HepG2.
Subcellular location: Cytosol.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:2,000
1:50-1:200
1:200
1:1,000
Uniprot #: SwissProt: P19429 Human | P23693 Rat
Alternative names: cardiac muscle Cardiac troponin I cardiomyopathy, dilated 2A (autosomal recessive) Cardiomyopathy, familial hypertrophic, 7, included CMD1FF CMD2A CMH7 cTnI Familial hypertrophic cardiomyopathy 7 MGC116817 RCM1 Tn1 Tni TNN I3 TNNC 1 TNNC1 TNNI3 TNNI3_HUMAN Troponin I Troponin I cardiac Troponin I cardiac muscle Troponin I cardiac muscle isoform Troponin I type 3 cardiac troponin I, cardiac 3 TroponinI Ttroponin I type 3 (cardiac)
Images
ET1702-37_1.jpg Fig1: Western blot analysis of Cardiac Troponin I on different lysates with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/2,000 dilution.

Lane 1: Rat heart tissue lysate
Lane 2: Rat skeletal muscle tissue lysate (negative)

Lysates/proteins at 30 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 30 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-37) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1702-37_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-37_3.jpg Fig3: Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin I with Rabbit anti-Cardiac Troponin I antibody (ET1702-37) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-37, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1702-37_4.jpg Fig4: ICC staining of Cardiac Troponin I in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.