ET1702-38

S100A10 Recombinant Rabbit Monoclonal Antibody [JF0987]

cat.: ET1702-38

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	JF0987

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 11 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human S100A10 aa 19-68 / 97.
Positive control:	A549 cell lysate, human lung tissue lysates, human lung tissue, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue.
Subcellular location:	Extracellular exosome.
Recommended Dilutions: WB IHC-P IF-Tissue	1:1,000-1:2,000 1:1,000-1:2,000 1:200
Uniprot #:	SwissProt: P60903 Human \| P08207 Mouse \| P05943 Rat
Alternative names:	42C AA409961 AL024248 Annexin II ligand Annexin II ligand, calpactin I, light polypeptide Annexin II tetramer (AIIt) p11 subunit Annexin II, light chain ANX2L ANX2LG Ca[1] CAL12 CAL1L Calpactin I light chain Calpactin I, p11 subunit Calpactin-1 light chain Cellular ligand of annexin II CLP11 GP11 MGC111133 Nerve growth factor-induced protein 42C OTTHUMP00000015269 OTTHUMP00000015270 p10 p10 protein p11 Protein S100 A10 Protein S100-A10 S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) S100 calcium binding protein A10 (calpactin) S100 calcium binding protein A10 S100 calcium-binding protein A10 S100a10 S10AA_HUMAN

Images

	Fig1: Western blot analysis of S100A10 on different lysates with Rabbit anti-S100A10 antibody (ET1702-38) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD S100A10 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Western blot analysis of S100A10 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
	Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-S100A10 antibody (ET1702-38) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-S100A10 antibody (ET1702-38) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-38) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-S100A10 antibody (ET1702-38) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-S100A10 antibody (ET1702-38) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-S100A10 antibody (ET1702-38) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling S100A10 with Rabbit anti-S100A10 antibody (ET1702-38) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-38, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.