Argonaute 2 Recombinant Rabbit Monoclonal Antibody [JF0992]
cat.: ET1702-39
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JF0992
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 97 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Argonaute 2 aa 361-404 / 859.
Positive control: HCT 116 cell lysate, HeLa cell lysate, Hela, MCF-7, AGS, mouse kidney tissue, mouse stomach tissue, human kidney tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:2,000
1:50-1:200
1:50-1:200
1:50-1:4,000
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9UKV8 Human | Q8CJG0 Mouse | Q9QZ81 Rat
Alternative names: Ago 2 AGO2_HUMAN Argonaute 2 argonaute 2, RISC catalytic component Argonaute RISC catalytic component 2 Argonaute2 CTA-204B4.6 dAgo2 eIF 2C 2 eIF-2C 2 eIF2C 2 Eif2c2 Eukaryotic translation initiation factor 2C 2 Eukaryotic translation initiation factor 2C subunit 2 hAgo2 MGC3183 PAZ Piwi domain protein PPD Protein argonaute-2 Protein slicer Q10 Slicer protein
Images
ET1702-39_1.jpg Fig1: All lanes: Western blot analysis of Ago2 with anti-Ago2 antibody [JF0992] (ET1702-39) at 1:500 dilution.

Lane 1: Wild-type CHO whole cell lysate.
Lane 2: Ago2 knockout CHO whole cell lysate.

ET1702-39 was shown to specifically react with Ago2 in wild-type CHO cells. No band was observed when Ago2 knockout samples were tested. Wild-type and Ago2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Ago2 antibody (ET1702-39, 1/500) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
ET1702-39_2.jpg Fig2: Western blot analysis of Argonaute 2 on different lysates with Rabbit anti-Argonaute 2 antibody (ET1702-39) at 1/2,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 97 kDa
Observed band size: 97 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-39) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1702-39_3.jpg Fig3: ICC staining of Argonaute 2 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-39_4.jpg Fig4: ICC staining of Argonaute 2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-39_5.jpg Fig5: ICC staining of Argonaute 2 in AGS cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-39_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Argonaute 2 antibody (ET1702-39) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-39) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-39_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Argonaute 2 antibody (ET1702-39) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-39) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-39_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Argonaute 2 antibody (ET1702-39) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-39) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-39_9.jpg Fig9: Flow cytometric analysis of Argonaute 2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.