ABCG2 Recombinant Rabbit Monoclonal Antibody [JF0994]
cat.: ET1702-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0994 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ABCG2 aa 140-180. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, HeLa cell lysate, human placenta tissue lysate, Hela cell lysate, 293T cell lysate, human ovarian cancer tissue, mouse kidney tissue. |
| Subcellular location: | Mitochondrion membrane, Cell membrane, Apical cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UNQ0 Human | Q7TMS5 Mouse |
| Alternative names: | ABC transporter ABC15 ABCG 2 ABCG2 ABCG2_HUMAN ABCP ATP binding cassette sub family G (WHITE) member 2 ATP binding cassette transporter G2 ATP-binding cassette sub-family G member 2 BCRP BCRP1 BMDP Breast cancer resistance protein CD338 CDw338 CDw338 antigen EST157481 GOUT1 MGC102821 Mitoxantrone resistance associated protein Mitoxantrone resistance-associated protein MRX Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 MXR MXR1 Placenta specific ATP binding cassette transporter Placenta specific MDR protein Placenta-specific ATP-binding cassette transporter UAQTL1 |
Images
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Fig1:
Western blot analysis of ABCG2 on different lysates with Rabbit anti-ABCG2 antibody (ET1702-40) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ABCG2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-40) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ABCG2 on different lysates with Rabbit anti-ABCG2 antibody (ET1702-40) at 1/2,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) Lane 2: Hep G2 (Human hepatocellular carcinoma cell) Lane 3: HeLa (Human cervical adenocarcinoma cell) Lysates/proteins at 20 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-40, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 72.3 kDa Observed band size: 72 kDa |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue with Rabbit anti-ABCG2 antibody (ET1702-40) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ABCG2 antibody (ET1702-40) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-40) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.