ABCG2 Recombinant Rabbit Monoclonal Antibody [JF0994]
cat.: ET1702-40
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JF0994
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 72 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ABCG2 aa 140-180.
Positive control: HepG2 cell lysate, human placenta tissue lysate, Hela cell lysate, 293T cell lysate, human ovarian cancer tissue, mouse kidney tissue, Hela, MCF-7, 293T.
Subcellular location: Mitochondrion membrane, Cell membrane, Apical cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UNQ0 Human | Q7TMS5 Mouse
Alternative names: ABC transporter ABC15 ABCG 2 ABCG2 ABCG2_HUMAN ABCP ATP binding cassette sub family G (WHITE) member 2 ATP binding cassette transporter G2 ATP-binding cassette sub-family G member 2 BCRP BCRP1 BMDP Breast cancer resistance protein CD338 CDw338 CDw338 antigen EST157481 GOUT1 MGC102821 Mitoxantrone resistance associated protein Mitoxantrone resistance-associated protein MRX Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 MXR MXR1 Placenta specific ATP binding cassette transporter Placenta specific MDR protein Placenta-specific ATP-binding cassette transporter UAQTL1
Images
ET1702-40_1.jpg Fig1: Western blot analysis of ABCG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: human placenta tissue lysate
Lane 3: Hela cell lysate
Lane 4: 293T cell lysate
ET1702-40_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue with Rabbit anti-ABCG2 antibody (ET1702-40) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-40_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ABCG2 antibody (ET1702-40) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-40) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-40_4.jpg Fig4: ICC staining of ABCG2 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-40_5.jpg Fig5: ICC staining of ABCG2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-40_6.jpg Fig6: ICC staining of ABCG2 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-40_7.jpg Fig7: Flow cytometric analysis of ABCG2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.