HMGCR Recombinant Rabbit Monoclonal Antibody [JF0981]
cat.: ET1702-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0981 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HMGCR aa 438-489 / 888. |
| Positive control: | HeLa cell lysate, THP-1 cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human small intestine tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:5,000 Use at an assay dependent concentration. 1:100 |
| Uniprot #: | SwissProt: P04035 Human | Q01237 Mouse | P51639 Rat |
| Alternative names: | 3 hydroxy 3 methylglutaryl CoA reductase 3 hydroxy 3 methylglutaryl Coenzyme A reductase 3 hydroxymethylglutaryl CoA reductase 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) 3-hydroxy-3-methylglutaryl-coenzyme A reductase 3H3M HMDH_HUMAN HMG CoA reductase HMG CoAR HMG-CoA reductase Hmgcr Hydroxymethylglutaryl CoA reductase LDLCQ3 MGC103269 Red |
Images
|
Fig1:
Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody (ET1702-41) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with ET1702-41 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1702-41 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of ET1702-41 in HeLa cell lysate Lane 3: ET1702-41 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody (ET1702-41) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-41) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.