Prealbumin Recombinant Rabbit Monoclonal Antibody [JF1016]
cat.: ET1702-42
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JF1016 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Prealbumin aa 11-50 / 147. |
| Positive control: | Human lung tissue lysates, human liver tissue, human pancreas tissue. |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P02766 Human |
| Alternative names: | Amyloid polyneuropathy Amyloidosis I ATTR Carpal tunnel syndrome 1 CTS CTS1 Dysprealbuminemic euthyroidal hyperthyroxinemia Dystransthyretinemic hyperthyroxinemia Epididymis luminal protein 111 HEL111 HsT2651 PALB Prealbumin amyloidosis type I Prealbumin Prealbumin Thyroxine-binding Senile systemic amyloidosis TBPA Thyroxine binding prealbumin Transthyretin TTHY_HUMAN TTR TTR protein |
Images
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Fig1: Western blot analysis of Prealbumin on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Prealbumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Prealbumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.