FOXP1 Recombinant Rabbit Monoclonal Antibody [JF1017]
cat.: ET1702-44
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JF1017
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 77/75 kDa
Isotype: IgG
Immunogen: Synthetic peptide within hunman FOXP1 aa 615-653 / 677.
Positive control: Hela cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human colon carcinoma tissue, human breast carcinoma tissue, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC
  IHC-P

1:1,000-1:5,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: Q9H334 Human | P58462 Mouse | Q498D1 Rat
Alternative names: 12CC4 FLJ23741 Fork head related protein like B Forkhead box P1 Forkhead box protein P1 FOX P1 FOXP 1 foxp1 FOXP1_HUMAN Glutamine rich factor 1 hFKH1B HSPC215 MGC12942 MGC88572 MGC99551 QRF 1 QRF1
Images
ET1702-44_1.jpg Fig1: Western blot analysis of FOXP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF-7 cell lysate
ET1702-44_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FOXP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-44_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-44_4.jpg Fig4: Flow cytometric analysis of FOXP1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-44, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.