Dysferlin Recombinant Rabbit Monoclonal Antibody [JF05-54]
cat.: ET1702-45
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JF05-54
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 237 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Dysferlin aa 113-162 / 2080.
Positive control: Skeletal muscle tissue lysates, SW480, human spleen tissue, mouse skeletal muscle tissue, mouse spleen tissue, mouse heart tissue.
Subcellular location: Cell membrane, Cytoplasmic vesicle membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O75923 Human | Q9ESD7 Mouse
Alternative names: DMAT DYSF DYSF_HUMAN Dysferlin Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) Dysferlin limb girdle muscular dystrophy 2B Dystrophy associated fer 1 like 1 Dystrophy associated fer 1 like protein Dystrophy associated fer1 like 1 Dystrophy associated fer1 like protein Dystrophy-associated fer-1-like protein Fer 1 like protein 1 Fer-1-like protein 1 Fer1 like protein 1 FER1L1 FLJ00175 FLJ90168 LGMD 2B LGMD2B Limb girdle muscular dystrophy 2B (autosomal recessive) Limb girdle muscular dystrophy 2B Miyoshi myopathy MM MMD1
Images
ET1702-45_1.jpg Fig1: Western blot analysis of Dysferlin on skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
ET1702-45_2.jpg Fig2: ICC staining of Dysferlin in SW480 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-45_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-45_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-45_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-45_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.