Dysferlin Recombinant Rabbit Monoclonal Antibody [JF05-54]
cat.: ET1702-45
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF05-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 237 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Dysferlin aa 113-162 / 2080. |
| Positive control: | Human skeletal muscle tissue lysates, human spleen tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000-1:5,000 1:50-1:200 1:200 |
| Uniprot #: | SwissProt: O75923 Human | Q9ESD7 Mouse |
| Alternative names: | DMAT DYSF DYSF_HUMAN Dysferlin Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) Dysferlin limb girdle muscular dystrophy 2B Dystrophy associated fer 1 like 1 Dystrophy associated fer 1 like protein Dystrophy associated fer1 like 1 Dystrophy associated fer1 like protein Dystrophy-associated fer-1-like protein Fer 1 like protein 1 Fer-1-like protein 1 Fer1 like protein 1 FER1L1 FLJ00175 FLJ90168 LGMD 2B LGMD2B Limb girdle muscular dystrophy 2B (autosomal recessive) Limb girdle muscular dystrophy 2B Miyoshi myopathy MM MMD1 |
Images
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Fig1:
Western blot analysis of Dysferlin on human skeletal muscle tissue lysates with Rabbit anti-Dysferlin antibody (ET1702-45) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 237 kDa Observed band size: 237 kDa Exposure time: 3 minutes; ECL: K1801; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-45) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Dysferlin antibody (ET1702-45) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Dysferlin antibody (ET1702-45) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.