CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]
cat.: ET1702-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JF10-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CNPase aa 386-421 / 421. |
| Positive control: | Mouse brain tissue lysate, mouse cerebrum tissue lysate, SHG-44, SH-SY5Y, N2A, mouse brain tissue, rat brain tissue, mouse cerebellum tissue. |
| Subcellular location: | Membrane, Melanosome. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000-1:2,000 1:500 1:100 |
| Uniprot #: | SwissProt: P09543 Human | P16330 Mouse | P13233 Rat |
| Alternative names: | 2' 2'3' cyclic nucleotide 3' phosphodiesterase 3'-cyclic-nucleotide 3'-phosphodiesterase CN37_HUMAN CNP 1 CNP CNP1 CNPase |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling CNPase with Rabbit anti-CNPase antibody (ET1702-46) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-46, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Western blot analysis of CNPase on different lysates with Rabbit anti-CNPase antibody (ET1702-46) at 1/2,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse liver tissue lysate (low expression) Lane 3: Rat cerebellum tissue lysate Lane 4: Rat liver tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-CNPase antibody (ET1702-46) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-46) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CNPase antibody (ET1702-46) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-46) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.