MHC class I Recombinant Rabbit Monoclonal Antibody [JF10-38]
cat.: ET1702-47
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JF10-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HLA aa 263-299 / 365. |
| Positive control: | HL-60 cell lysate, K-562 cell lysate, SH-SY5Y, human spleen tissue, THP-1. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:2,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P04439 Human | P01889 Human |
| Alternative names: | A 28 A 9 Antigen presenting molecule Aw 24 Aw 68 CLASS I HISTOCOMPATIBILITY ANTIGEN H2 K1 H2K HLA A HLA class I histocompatibility antigen A 1 alpha chain HLA class I histocompatibility antigen A 11 alpha chain HLA class I histocompatibility antigen A 2 alpha chain HLA class I histocompatibility antigen A 24 alpha chain HLA class I histocompatibility antigen A 3 alpha chain HLA class I histocompatibility antigen A 30 alpha chain HLA class I histocompatibility antigen A 32 alpha chain HLA class I histocompatibility antigen A 68 alpha chain HLAA Leukocyte antigen class I A Major histocompatibility complex class I A MHC class I antigen A 1 MHC class I antigen A*11 MHC class I antigen A*2 MHC class I antigen A*24 MHC class I antigen A*3 MHC class I antigen A*30 MHC class I antigen A*32 MHC class I antigen A*68 MHC class I antigen HLA A heavy chain MHC class I heavy chain H2 K |
Images
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Fig1:
Western blot analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody (ET1702-47) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: K-562 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-47) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling MHC class I with Rabbit anti-MHC class I antibody (ET1702-47) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MHC class I antibody (ET1702-47) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of MHC class I was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-47, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.