ET1702-48

Galectin 3 Recombinant Rabbit Monoclonal Antibody [JF39-10]

cat.: ET1702-48

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, FC, IHC-P
Clonality:	Monoclonal
Clone number:	JF39-10

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 26 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human Galectin 3 aa 1-49 / 250.
Positive control:	NIH/3T3 cell lysate, Hela cell lysate, THP-1 cell lysate, HeLa, human thyroid carcinoma tissue, human colon tissue, rat bladder tissue.
Subcellular location:	Nucleus, Secreted, Cytoplasm.
Recommended Dilutions: WB IF-Cell IF-Tissue FC IHC-P	1:500-1:2,000 1:50-1:200 1:50-1:200 1:1,000 1:200-1:1,000
Uniprot #:	SwissProt: P17931 Human \| P16110 Mouse \| P08699 Rat
Alternative names:	35 kDa lectin Carbohydrate binding protein 35 Carbohydrate-binding protein 35 CBP 35 CBP35 Gal-3 GAL3 Galactose-specific lectin 3 Galactoside-binding protein GALBP Galectin 3 internal gene,included Galectin-3 Galectin 3 Galectin3 GALIG GBP IgE binding protein IgE-binding protein L 31 L 34 L-31 L-34 galactoside-binding lectin L31 Laminin-binding protein Lectin L-29 Lectin, galactose binding, soluble 3 LEG3_HUMAN LGALS2 LGALS3 MAC 2 antigen Mac-2 Mac-2 antigen MAC2 Macrophage galactose-specific lectin MGC105387

Images

	Fig1: Western blot analysis of Galectin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Hela cell lysate Lane 3: THP-1 cell lysate
	Fig2: Western blot analysis of Galectin 3 on different lysates with Rabbit anti-Galectin 3 antibody (ET1702-48) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig3: Immunocytochemistry analysis of HeLa cells labeling Galectin 3 with Rabbit anti-Galectin 3 antibody (ET1702-48) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galectin 3 antibody (ET1702-48) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

	Fig4: Flow cytometric analysis of HeLa cells labeling Galectin 3. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-48, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
	Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Galectin 3 antibody (ET1702-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Galectin 3 antibody (ET1702-48) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-48) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Galectin 3 antibody (ET1702-48) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.