ET1702-49

PDGFR alpha Recombinant Rabbit Monoclonal Antibody [JF104-6]

cat.: ET1702-49

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IHC-P, IHC-Fr
Clonality:	Monoclonal
Clone number:	JF104-6

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 123 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within C-terminal human PDGFR alpha.
Positive control:	Mouse embryonic femur tissue, mouse embryonic intervertebral disc tissue, mouse embryonic lung tissue, rat embryonic femur tissue, rat embryonic intervertebral disc tissue, rat embryonic lung tissue, rat endometrium tissue, human colon tissue, human colon cancer tissue, human glioblastoma tissue, NIH/3T3 cell lysates, SHG-44 cell lysates, A549, NIH/3T3.
Subcellular location:	Golgi apparatus, Cell membrane, cilium.
Recommended Dilutions: WB IHC-P IHC-Fr	1:5,000-1:10,000 1:500-1:2,000 1:500
Uniprot #:	SwissProt: P16234 Human \| P26618 Mouse \| P20786 Rat
Alternative names:	Alpha-type platelet-derived growth factor receptor CD140 antigen-like family member A CD140a CD140a antigen MGC74795 PDGF alpha chain PDGF-R-alpha PDGFR 2 PDGFR alpha PDGFR2 PDGFRA PDGFRA/BCR fusion PGFRA_HUMAN Platelet derived growth factor receptor 2 Platelet derived growth factor receptor alpha Platelet derived growth factor receptor alpha polypeptide Platelet derived growth factor receptor Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein RHEPDGFRA

Images

	Fig1: Application: IHC-Fr Species: Mouse Site: E14.5 embyro Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
	Fig2: Immunohistochemical analysis of paraffin-embedded mouse embryonic femur tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse embryonic intervertebral disc tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded mouse embryonic lung tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat embryonic femur tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat embryonic intervertebral disc tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig7: Immunohistochemical analysis of paraffin-embedded rat embryonic lung tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded rat endometrium tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig9: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig10: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig11: Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig12: Western blot analysis of PDGFR alpha on NIH/3T3 cell lysates with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 123 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-49) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig13: Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/5,000 dilution.

Lane 1: NIH/3T3-si NT cell lysate
Lane 2: NIH/3T3-si PDGFR alpha cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 123 kDa
Observed band size: 190 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-49, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.