PDGFR alpha Recombinant Rabbit Monoclonal Antibody [JF104-6]
cat.: ET1702-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JF104-6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 123 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human PDGFR alpha. |
| Positive control: | Mouse embryonic femur tissue, NIH/3T3 cell lysates. |
| Subcellular location: | Golgi apparatus, Cell membrane, cilium. |
| Recommended Dilutions:
WB IHC-Fr |
1:5,000-1:10,000 1:500 |
| Uniprot #: | SwissProt: P16234 Human | P26618 Mouse | P20786 Rat |
| Alternative names: | Alpha-type platelet-derived growth factor receptor CD140 antigen-like family member A CD140a CD140a antigen MGC74795 PDGF alpha chain PDGF-R-alpha PDGFR 2 PDGFR alpha PDGFR2 PDGFRA PDGFRA/BCR fusion PGFRA_HUMAN Platelet derived growth factor receptor 2 Platelet derived growth factor receptor alpha Platelet derived growth factor receptor alpha polypeptide Platelet derived growth factor receptor Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein RHEPDGFRA |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: E14.5 embyro Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Western blot analysis of PDGFR alpha on NIH/3T3 cell lysates with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 123 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-49) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/5,000 dilution. Lane 1: NIH/3T3-si NT cell lysate Lane 2: NIH/3T3-si PDGFR alpha cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 123 kDa Observed band size: 190 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-49, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.