NQO1 Recombinant Rabbit Monoclonal Antibody [JF440-1]
cat.: ET1702-50
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF440-1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NQO1 aa 1-49 / 274. |
| Positive control: | HCT 116 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human breast cancer tissue, MCF7. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP FC IHC-P |
1:1,000-1:5,000 Use at an assay dependent concentration. 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P15559 Human | Q64669 Mouse | P05982 Rat |
| Alternative names: | Azoreductase Cytochrome b 5 reductase DHQU DIA 4 DIA4 Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) Diaphorase (NADH/NADPH) Diaphorase 4 Dioxin inducible 1 DT diaphorase DT-diaphorase DTD Menadione reductase NAD(P)H dehydrogenase [quinone] 1 NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 NAD(P)H:menadione oxidoreductase 1 NAD(P)H:Quinone acceptor oxidoreductase type 1 NAD(P)H:quinone oxidoreductase 1 NAD(P)H:quinone oxireductase NMOR 1 NMOR I NMOR1 NMORI NQO 1 NQO1 NQO1_HUMAN Phylloquinone reductase QR 1 QR1 Quinone reductase 1 |
Images
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Fig1:
Western blot analysis of NQO1 on different lysates with Rabbit anti-NQO1 antibody (ET1702-50) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si NQO1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-50) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of NQO1 on different lysates with Rabbit anti-NQO1 antibody (ET1702-50) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-NQO1 antibody (ET1702-50) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-50) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of MCF7 cells labeling NQO1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-50, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.