NQO1 Recombinant Rabbit Monoclonal Antibody [JF440-1]
cat.: ET1702-50
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JF440-1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NQO1 aa 1-49 / 274. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, C2C12 cell lysate, mouse kidney tissue lysate, mouse heart tissue lysate, Hela. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:1,000-1:5,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P15559 Human | Q64669 Mouse | P05982 Rat |
| Alternative names: | Azoreductase Cytochrome b 5 reductase DHQU DIA 4 DIA4 Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) Diaphorase (NADH/NADPH) Diaphorase 4 Dioxin inducible 1 DT diaphorase DT-diaphorase DTD Menadione reductase NAD(P)H dehydrogenase [quinone] 1 NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 NAD(P)H:menadione oxidoreductase 1 NAD(P)H:Quinone acceptor oxidoreductase type 1 NAD(P)H:quinone oxidoreductase 1 NAD(P)H:quinone oxireductase NMOR 1 NMOR I NMOR1 NMORI NQO 1 NQO1 NQO1_HUMAN Phylloquinone reductase QR 1 QR1 Quinone reductase 1 |
Images
|
Fig1:
Western blot analysis of NQO1 on different lysates with Rabbit anti-NQO1 antibody (ET1702-50) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NQO1 on different lysates with Rabbit anti-NQO1 antibody (ET1702-50) at 1/1,000 dilution. Lane 1: C2C12 cell lysate (10 µg/Lane) Lane 2: Mouse kidney tissue lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (20 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Flow cytometric analysis of NQO1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.