Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, ICC/IF, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JF40-10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Protein A affinity purified. |
Molecular weight: | 34 kDa |
Isotype: | IgG |
Immunogen: | recombinant protein |
Positive control: | Raji, 293T, Hela, HepG2, human tonsil tissue, human spleen tissue. |
Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome, Lysosome. |
Recommended Dilutions:
WB ICC/IF IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P04233 Human |
Alternative names: | CD 74 antibody CD74 antibody CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody CD74 antigen antibody CD74 molecule antibody CD74 molecule, major histocompatibility complex, class II invariant chain antibody CLIP antibody DHLAG antibody Gamma chain of class II antigens antibody HG2A_HUMAN antibody HLA class II histocompatibility antigen gamma chain antibody HLA DR antigens associated invariant chain antibody HLA DR gamma antibody HLA-DR antigens-associated invariant chain antibody HLA-DR-gamma antibody HLADG antibody HLADR antigens associated invariant chain antibody Ia antigen associated invariant chain antibody Ia antigen-associated invariant chain antibody Ia associated invariant chain antibody Ia gamma antibody Ii antibody Invariant polypeptide of major histocompatibility complex class II antigen associated antibody la-gamma antibody Major histocompatibility complex class II invariant chain antibody MHC HLA DR gamma chain antibody MHC HLA-DR gamma chain antibody p33 antibody p35 antibody Protein 41 antibody |
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Fig1: Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution. |
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Fig2: ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD74 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |