ET1702-51

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, ICC/IF, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JF40-10

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1 mg/mL.

Purification:

Protein A affinity purified.

Molecular weight:

34 kDa

Isotype:

IgG

Immunogen:

recombinant protein

Positive control:

Raji, 293T, Hela, HepG2, human tonsil tissue, human spleen tissue.

Subcellular location:

Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome, Lysosome.

Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: P04233 Human

Alternative names:

CD 74 antibody CD74 antibody CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody CD74 antigen antibody CD74 molecule antibody CD74 molecule, major histocompatibility complex, class II invariant chain antibody CLIP antibody DHLAG antibody Gamma chain of class II antigens antibody HG2A_HUMAN antibody HLA class II histocompatibility antigen gamma chain antibody HLA DR antigens associated invariant chain antibody HLA DR gamma antibody HLA-DR antigens-associated invariant chain antibody HLA-DR-gamma antibody HLADG antibody HLADR antigens associated invariant chain antibody Ia antigen associated invariant chain antibody Ia antigen-associated invariant chain antibody Ia associated invariant chain antibody Ia gamma antibody Ii antibody Invariant polypeptide of major histocompatibility complex class II antigen associated antibody la-gamma antibody Major histocompatibility complex class II invariant chain antibody MHC HLA DR gamma chain antibody MHC HLA-DR gamma chain antibody p33 antibody p35 antibody Protein 41 antibody

	Fig1: Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution.
	Fig2: ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: ICC staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD74 antibody. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin.
	Fig7: Flow cytometric analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.