CD74 Recombinant Rabbit Monoclonal Antibody [JF40-10]
cat.: ET1702-51
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JF40-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD74 aa 21-64 / 296. |
| Positive control: | Raji, 293T, Hela, HepG2, human tonsil tissue, human spleen tissue, human lymph nodes tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, trans-Golgi network, cell membrane, lysosome, endosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P04233 Human |
| Alternative names: | CD 74 CD74 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) CD74 antigen CD74 molecule CD74 molecule, major histocompatibility complex, class II invariant chain CLIP DHLAG Gamma chain of class II antigens HG2A_HUMAN HLA class II histocompatibility antigen gamma chain HLA DR antigens associated invariant chain HLA DR gamma HLA-DR antigens-associated invariant chain HLA-DR-gamma HLADG HLADR antigens associated invariant chain Ia antigen associated invariant chain Ia antigen-associated invariant chain Ia associated invariant chain Ia gamma Ii Invariant polypeptide of major histocompatibility complex class II antigen associated la-gamma Major histocompatibility complex class II invariant chain MHC HLA DR gamma chain MHC HLA-DR gamma chain p33 p35 Protein 41 |
Images
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Fig1: Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution. |
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Fig2: ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD74 antibody (ET1702-51) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-51) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD74 antibody (ET1702-51) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-51) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.