Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat, Zebrafish |
Applications: | WB, IP, IHC-P |
Clonality: | Monoclonal |
Clone number: | JF47-01 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 42 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Actin aa 303-349 / 377. |
Positive control: | Zebrafish tissue lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, hybrid fish (crucian-carp) brain tissue lysate, hybrid fish (crucian-carp) kidney tissue lysate, human fetal skeletal muscle tissue, mouse skeletal muscle tissue. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB IP |
1:5,000-1:10,000 1:50-1:200 |
Uniprot #: | SwissProt: P68133 Human | P68134 Mouse | P68136 Rat |
Alternative names: | a actin ACTA ACTA1 Actin alpha skeletal muscle Actin actin, alpha 1, skeletal muscle 1 actin, alpha 1, skeletal muscle Actin, alpha skeletal muscle actina actine ACTS_HUMAN aktin Alpha Actin 1 Alpha skeletal muscle Actin alpha skeletal muscle alpha-actin Alpha-actin-1 ASMA CFTD CFTD1 CFTDM MPFD NEM1 NEM2 NEM3 nemaline myopathy type 3 |
Fig1:
Western blot analysis of Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Zebrafish tissue lysate Lane 2: Hela cell lysate Lane 3: PC-12 cell lysate Lane 4: NIH/3T3 cell lysate |
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Fig2:
Western blot analysis of Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hybrid fish (crucian-carp) brain tissue lysate Lane 2: Hybrid fish (crucian-carp) kidney tissue lysate |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-Actin antibody (ET1702-52) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-52) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Actin antibody (ET1702-52) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-52) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |