Actin Recombinant Rabbit Monoclonal Antibody [JF47-01]
cat.: ET1702-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IP, IHC-P
Clonality: Monoclonal
Clone number: JF47-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Actin aa 303-349 / 377.
Positive control: Zebrafish tissue lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, hybrid fish (crucian-carp) brain tissue lysate, hybrid fish (crucian-carp) kidney tissue lysate, human fetal skeletal muscle tissue, mouse skeletal muscle tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IP

1:5,000-1:10,000
1:50-1:200
Uniprot #: SwissProt: P68133 Human | P68134 Mouse | P68136 Rat
Alternative names: a actin ACTA ACTA1 Actin alpha skeletal muscle Actin actin, alpha 1, skeletal muscle 1 actin, alpha 1, skeletal muscle Actin, alpha skeletal muscle actina actine ACTS_HUMAN aktin Alpha Actin 1 Alpha skeletal muscle Actin alpha skeletal muscle alpha-actin Alpha-actin-1 ASMA CFTD CFTD1 CFTDM MPFD NEM1 NEM2 NEM3 nemaline myopathy type 3
Images
ET1702-52_1.jpg Fig1: Western blot analysis of Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Zebrafish tissue lysate
Lane 2: Hela cell lysate
Lane 3: PC-12 cell lysate
Lane 4: NIH/3T3 cell lysate
ET1702-52_2.jpg Fig2: Western blot analysis of Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hybrid fish (crucian-carp) brain tissue lysate
Lane 2: Hybrid fish (crucian-carp) kidney tissue lysate
ET1702-52_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-Actin antibody (ET1702-52) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-52) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-52_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Actin antibody (ET1702-52) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-52) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.