TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024]
cat.: ET1702-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JF1-024 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 95 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TGN46 aa 43-79 / 437L5L562:L576. |
| Positive control: | NIH/3T3 cell lysate, Hela cell lysate, human kidney tissue, human liver tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus. |
| Recommended Dilutions:
WB IP IHC-P |
1:1,000-1:2,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: O43493 Human | Q62314 Mouse |
| Alternative names: | TGN 46 TGN 51 TGN38 TGN38 homolog TGN46 TGN48 TGN51 TGOLN2 TGON2_HUMAN Trans Golgi network integral membrane protein 2 Trans golgi network protein 2 Trans Golgi network protein TGN51 Trans-Golgi network integral membrane protein 2 Trans-Golgi network protein TGN51 TTGN2 |
Images
|
Fig1:
Western blot analysis of TGN46 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Hela cell lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.