ET1702-56

TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024]

cat.: ET1702-56

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, IP, IF-Cell, FC
Clonality:	Monoclonal
Clone number:	JF1-024

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	95 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human TGN46 aa 43-79 / 437L5L562:L576.
Positive control:	NIH/3T3 cell lysate, Hela cell lysate, human kidney tissue, human liver tissue.
Subcellular location:	Cell membrane, Golgi apparatus.
Recommended Dilutions: WB IP IHC-P IF-Cell FC	1:1,000-1:2,000 1:50-1:100 1:50-1:200 1:100 1:1000
Uniprot #:	SwissProt: O43493 Human \| Q62314 Mouse
Alternative names:	TGN 46 TGN 51 TGN38 TGN38 homolog TGN46 TGN48 TGN51 TGOLN2 TGON2_HUMAN Trans Golgi network integral membrane protein 2 Trans golgi network protein 2 Trans Golgi network protein TGN51 Trans-Golgi network integral membrane protein 2 Trans-Golgi network protein TGN51 TTGN2

Images

	Fig1: Western blot analysis of TGN46 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-56, 1/1,000) was used in 5% NFDM/TBST at 4° overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-56, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TGN46 antibody (ET1702-56) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1702-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig5: Immunocytochemistry analysis of HepG2 cells labeling TGN46 with Rabbit anti-TGN46 antibody (ET1702-56) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TGN46 antibody (ET1702-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Fig6: Flow cytometric analysis of HepG2 cells labeling TGN46.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-56, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.