CD147 Recombinant Rabbit Monoclonal Antibody [JF1-045]
cat.: ET1702-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JF1-045
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD147 aa 171-204 / 385.
Positive control: HepG2 cell lysate, NCI-H226 cell lysate, 786-0 cell lysate, RAW264.7 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, Hela, A431, SKOV-3, human colon carcinoma tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, mouse testis tissue.
Subcellular location: Cell membrane, Melanosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:5,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P35613 Human | P18572 Mouse | P26453 Rat
Alternative names: 5A11 antigen 5F7 BASI_HUMAN Basigin (Ok blood group) Basigin Blood brain barrier HT7 antigen Bsg CD 147 CD147 CD147 antigen Collagenase stimulatory factor EMMPRIN Extracellular matrix metalloproteinase inducer Leukocyte activation antigen M6 M 6 M6 M6 leukocyte activation antigen Neurothelin OK OK blood group OK blood group antigen TCSF Tumor cell derived collagenase stimulatory factor Tumor cell-derived collagenase stimulatory factor
Images
ET1702-58_1.jpg Fig1: Western blot analysis of CD147 on different lysates with Rabbit anti-CD147 antibody (ET1702-58) at 1/5,000 dilution.

Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: NCI-H226 cell lysate (15 µg/Lane)
Lane 3: 786-0 cell lysate (15 µg/Lane)
Lane 4: RAW264.7 cell lysate (15 µg/Lane)
Lane 5: Mouse liver tissue lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (20 µg/Lane)

Predicted band size: 42 kDa
Observed band size: 45-60 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1702-58_2.jpg Fig2: All lanes: Western blot analysis of CD147 with anti-CD147 antibody[JF1-045] (ET1702-58) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2/3: CD147 knockdown Hela whole cell lysate (10 µg).

ET1702-58 was shown to specifically react with CD147 in wild-type Hela cells. Weakened bands were observed when CD147 knockdown samples were tested. Wild-type and CD147 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1702-58_3.jpg Fig3: ICC staining of CD147 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-58_4.jpg Fig4: ICC staining of CD147 in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-58_5.jpg Fig5: ICC staining of CD147 in SKOV-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-58_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD147 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-58_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-58_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-58_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-58_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.