HE4 Recombinant Rabbit Monoclonal Antibody [JF62-09]
cat.: ET1702-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JF62-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HE4 aa 78-114 / 124. |
| Positive control: | SW480 cell lysate, HepG2 cell lysate, OVCAR-3 cell lysate, mouse lung tissue lysate, mouse kidney tissue lysate, HepG2, mouse lung tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q14508 Human | Q9DAU7 Mouse |
| Alternative names: | dJ461P17.6 EDDM4 epididymal protein 4 Epididymal secretory protein E4 Epididymis specific whey acidic protein type four disulfide core HE 4 Major epididymis specific protein E4 Major epididymis-specific protein E4 MGC57529 Putative protease inhibitor WAP5 WAP 5 WAP domain containing protein HE4 WAP domain containing protein HE4 V4 WAP four disulfide core domain 2 WAP four disulfide core domain protein 2 WAP four-disulfide core domain protein 2 WAP5 WFDC 2 WFDC2 WFDC2_HUMAN |
Images
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Fig1:
Western blot analysis of HE4 on different lysates with Rabbit anti-HE4 antibody (ET1702-61) at 1/2,000 dilution. Lane 1: SW480 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: OVCAR-3 cell lysate (15 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 13 kDa Observed band size: 20 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-61) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling HE4 with Rabbit anti-HE4 antibody (ET1702-61) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HE4 antibody (ET1702-61) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling HE4. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-61, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.