Annexin V Recombinant Rabbit Monoclonal Antibody [JF50-11]
cat.: ET1702-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF50-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Annexin V aa 281-320 / 320. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Human liver tissue lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse liver tissue lysate, C6 cell lysate, PC-12 cell lysate, A549 cell lysate. |
| Subcellular location: | Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P08758 Human | P48036 Mouse | P14668 Rat |
| Alternative names: | Anchorin CII Annexin 5 Annexin A5 Annexin V Annexin-5 ANX A5 ANX5 ANXA5 ANXA5_HUMAN Calphobindin I CBP-I Endonexin II ENX2 Lipocortin V PAP I PAP-I Placental anticoagulant protein 4 Placental anticoagulant protein I PLACENTAL PROTEIN 4 Pp4 Thromboplastin inhibitor VAC-alpha Vascular anticoagulant-alpha |
Images
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Fig1:
Western blot analysis of Annexin V on different lysates with Rabbit anti-Annexin V antibody (ET1702-62) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Human liver tissue lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: C6 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Annexin V on different lysates with Rabbit anti-Annexin V antibody (ET1702-62) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Annexin V cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 30 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-62) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.