Anti-Annexin V antibody [JF50-11]
cat.: ET1702-62
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, FC
Clonality: Monoclonal
Clone number: JF50-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 36 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Annexin V.
Positive control: Hela cell lysate, HepG2 cell lysate, Hela, A431.
Subcellular location: Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane.
Recommended Dilutions:
  WB
  FC
  ICC/IF

1:500-1:2,000
1:50-1:100
1:100-1:500
Uniprot #: SwissProt: P08758 Human | P48036 Mouse | P14668 Rat
Alternative names: Anchorin CII antibody Annexin 5 antibody Annexin A5 antibody Annexin V antibody Annexin-5 antibody ANX A5 antibody ANX5 antibody ANXA5 antibody ANXA5_HUMAN antibody Calphobindin I antibody CBP-I antibody Endonexin II antibody ENX2 antibody Lipocortin V antibody PAP I antibody PAP-I antibody Placental anticoagulant protein 4 antibody Placental anticoagulant protein I antibody PLACENTAL PROTEIN 4 antibody Pp4 antibody Thromboplastin inhibitor antibody VAC-alpha antibody Vascular anticoagulant-alpha antibody
Images
ET1702-62_1.jpg Fig1: Western blot analysis of Annexin V on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
ET1702-62_2.jpg Fig2: ICC staining of Annexin V in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-62_3.jpg Fig3: ICC staining of Annexin V in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-62_4.jpg Fig4: Flow cytometric analysis of Annexin V was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-62, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.