Annexin V Recombinant Rabbit Monoclonal Antibody [JF50-11]
cat.: ET1702-62
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: JF50-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Annexin V aa 281-320 / 320.
Positive control: HeLa cell lysate, HepG2 cell lysate, human liver tissue lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse liver tissue lysate, C6 cell lysate, PC-12 cell lysate, Hela.
Subcellular location: Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane.
Recommended Dilutions:
  WB
  FC

1:1,000
1:50-1:100
Uniprot #: SwissProt: P08758 Human | P48036 Mouse | P14668 Rat
Alternative names: Anchorin CII Annexin 5 Annexin A5 Annexin V Annexin-5 ANX A5 ANX5 ANXA5 ANXA5_HUMAN Calphobindin I CBP-I Endonexin II ENX2 Lipocortin V PAP I PAP-I Placental anticoagulant protein 4 Placental anticoagulant protein I PLACENTAL PROTEIN 4 Pp4 Thromboplastin inhibitor VAC-alpha Vascular anticoagulant-alpha
Images
ET1702-62_1.jpg Fig1: Western blot analysis of Annexin V on different lysates with Rabbit anti-Annexin V antibody (ET1702-62) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Human liver tissue lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: C6 cell lysate
Lane 8: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1702-62_2.jpg Fig2: Flow cytometric analysis of Annexin V was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-62, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.