Midkine Recombinant Rabbit Monoclonal Antibody [JF096-5]
cat.: ET1702-64
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JF096-5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Midkine aa 100-143 / 143. |
| Positive control: | HepG2 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, human liver tissue, human pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P21741 Human |
| Alternative names: | Amphiregulin associated protein Amphiregulin-associated protein ARAP FLJ27379 Mdk Midgestation and kidney protein Midkine MK 1 MK MK_HUMAN MK1 NEGF 2 NEGF2 Neurite growth promoting factor 2 Neurite outgrowth promoting protein Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein Retinoic acid inducible factor |
Images
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Fig1:
Western blot analysis of Midkine on different lysates with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.