p38 alpha / MAPK14 Recombinant Rabbit Monoclonal Antibody [JF55-07]
cat.: ET1702-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JF55-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human MAPK14. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC |
1:5,000 1:100 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q16539 Human | P47811 Mouse | P70618 Rat |
| Alternative names: | CSAID-binding protein Csaids binding protein CSBP CSBP1 CSBP2 CSPB1 Cytokine suppressive anti-inflammatory drug-binding protein EXIP MAP kinase 14 MAP kinase MXI2 MAP kinase p38 alpha MAPK 14 MAPK14 MAX-interacting protein 2 Mitogen-activated protein kinase 14 Mitogen-activated protein kinase p38 alpha MK14_HUMAN Mxi2 p38 p38 MAP kinase p38 mitogen activated protein kinase p38ALPHA p38alpha Exip PRKM14 PRKM15 RK SAPK2A Stress-activated protein kinase 2a |
Images
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Fig1:
Western blot analysis of p38 alpha / MAPK14 on different lysates with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 4: C2C12 (Mouse myoblast) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat kidney tissue lysate Cell Lysates/proteins at 15 µg/Lane, Tissue Lysates/proteins at 30 µg/Lane Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-65, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 38 kDa Observed band size: 41 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: ET1702-65, 1/50, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Application: Flow Cytometry (Intra) Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: ET1702-65(1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.