HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody [JF81-04]
cat.: ET1702-66
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey, Chicken, Fish |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF81-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Human GAPDH aa 1-335 / 335. |
| Positive control: | HeLa cell lysate, L-929 cell lysate, RAW264.7 cell lysate, C6 cell lysate, COS-1 cell lysate, rat heart tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane. |
| Recommended Dilutions:
WB |
1:50,000 |
| Uniprot #: | SwissProt: P04406 Human | P16858 Mouse | P04797 Rat |
| Alternative names: | 38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10 |
Images
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Fig1:
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (ET1702-66) at 1/50,000 dilution. Lane 1: HeLa cell lysate Lane 2: L-929 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lane 6: Rat heart tissue lysate Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-66) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.