HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]
cat.: ET1702-67
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish, Monkey, Hamster, Arabidopsis thaliana, Rice |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF53-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Human beta Actin aa 1-375 / 375. |
| Positive control: | HeLa cell lysate, A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, hybrid fish (crucian-carp) brain tissue lysates. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: P60709 Human | P60710 Mouse | P60711 Rat |
| Alternative names: | A26C1A A26C1B ACTB ACTB_HUMAN Actin beta Actin cytoplasmic 1 Actin, cytoplasmic 1, N-terminally processed Actx b actin Beta cytoskeletal actin Beta-actin BRWS1 E430023M04Rik MGC128179 PS1TP5 binding protein 1 PS1TP5BP1 β-actin β actin |
Images
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Fig1:
Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (ET1702-67) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: Jurkat cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lane 8: Mouse lung tissue lysate Lane 9: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-67) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
|
Fig2: Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.