HRP Conjugated beta Tubulin Recombinant Rabbit Monoclonal Antibody [JF41-50]
cat.: ET1702-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF41-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human beta Tubulin aa 308-357 / 444. |
| Positive control: | Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB |
1:10,000-1:50,000 |
| Uniprot #: | SwissProt: P07437 Human | P99024 Mouse | P69897 Rat |
| Alternative names: | Beta 4 tubulin Beta 5 tubulin BetaTubulin TBB5_HUMAN TUBB TUBB2 TUBB2A TUBB5 tubulin beta 2A Tubulin beta chain Tubulin beta-5 chain |
Images
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Fig1:
Western blot analysis of beta Tubulin on different lysates with Rabbit anti-beta Tubulin antibody (ET1702-68) at 1/10,000 dilution. Lane 1: HeLa Lane 2: HEK-293 Lane 3: NIH/3T3 Lane 4: RAW264.7 Lane 5: C6 Lane 6: PC-12 Lane 7: COS-1 Lysates/proteins at 20 µg/Lane. Exposure time: 6s; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-68, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 49.7 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.