| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF41-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human beta Tubulin aa 308-357 / 444. |
| Positive control: | Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB |
1:10,000-1:50,000 |
| Uniprot #: | SwissProt: P07437 Human | P99024 Mouse | P69897 Rat |
| Alternative names: | Beta 4 tubulin Beta 5 tubulin BetaTubulin TBB5_HUMAN TUBB TUBB2 TUBB2A TUBB5 tubulin beta 2A Tubulin beta chain Tubulin beta-5 chain |
|
Fig1:
Western blot analysis of beta Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-68, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Positive control: Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate |