HRP Conjugated GFP Recombinant Rabbit Monoclonal Antibody [JF51-05]
cat.: ET1702-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF51-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Aequorea victoria GFP. |
| Positive control: | 293T transfected with GFP cell lysates, K-562 transfected with GFP cell lysates. |
| Recommended Dilutions:
WB |
1:10,000-1:40,000 |
| Uniprot #: | SwissProt: P42212 Aequorea victoria |
| Alternative names: | GFP Green fluorescent protein yfp |
Images
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Fig1:
Western blot analysis of GFP on 293T transfected with GFP cell lysates with Rabbit anti-GFP antibody (ET1702-69) at 1/40,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-69) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
|
Fig2:
Western blot analysis of GFP on K-562 transfected with GFP cell lysates with Rabbit anti-GFP antibody (ET1702-69) at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 120% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-69) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.