PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
cat.: ET1702-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF97-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PDIA2 aa 405-450. |
| Positive control: | NIH/3T3 cell lysate, human liver tissue lysate, Hela. |
| Subcellular location: | Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q13087 Human | D3Z6P0 Mouse Unigene: 65154 Rat |
| Alternative names: | Pancreas specific protein disulfide isomerase Pancreas-specific protein disulfide isomerase Pancreatic protein disulfide isomerase PDA2 PDI PDIA2 PDIA2_HUMAN PDIp PDIR Protein disulfide isomerase A2 Protein disulfide isomerase Protein disulfide isomerase associated 2 Protein disulfide isomerase family A member 2 Protein disulfide isomerase pancreatic Protein disulfide-isomerase A2 Rho GDP dissociation inhibitor gamma |
Images
|
Fig1:
Western blot analysis of PDIA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Human liver tissue lysate Predicted band size: 57 kDa Observed band size: 57 kDa |
|
Fig2: Flow cytometric analysis of PDIA2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-70, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.