PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
cat.: ET1702-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF97-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PDIA2 aa 405-450. |
| Positive control: | HEK-293 cell lysate, NIH/3T3 cell lysate, Human liver tissue lysate. |
| Subcellular location: | Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q13087 Human | D3Z6P0 Mouse |
| Alternative names: | Pancreas specific protein disulfide isomerase Pancreas-specific protein disulfide isomerase Pancreatic protein disulfide isomerase PDA2 PDI PDIA2 PDIA2_HUMAN PDIp PDIR Protein disulfide isomerase A2 Protein disulfide isomerase Protein disulfide isomerase associated 2 Protein disulfide isomerase family A member 2 Protein disulfide isomerase pancreatic Protein disulfide-isomerase A2 Rho GDP dissociation inhibitor gamma |
Images
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Fig1:
Western blot analysis of PDIA2 on different lysates with Rabbit anti-PDIA2 antibody (ET1702-70) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human liver tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: Lane 1: 1 minute 18 seconds; Lane 2-3: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.