CD19 Recombinant Rabbit Monoclonal Antibody [JF099-9]
cat.: ET1702-74
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF099-9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD19 aa 384-430 / 556. |
| Positive control: | Raji cell lysate, Ramos cell lysate, PC-12 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Jurkat. |
| Subcellular location: | Membrane, Membrane raft. |
| Recommended Dilutions:
WB FC |
1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P15391 Human | P25918 Mouse Entrez Gene: 365367 Rat |
| Alternative names: | deficiency due to defect in CD19, included AW495831 B lymphocyte antigen CD19 B lymphocyte surface antigen B4 B-lymphocyte antigen CD19 B-lymphocyte surface antigen B4 B4 CD19 CD19 antigen CD19 molecule Cd19 protein CD19_HUMAN CVID3 Differentiation antigen CD19 Leu 12 Leu-12 Leu12 MGC109570 MGC12802 T-cell surface antigen Leu-12 |
Images
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Fig1:
Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (ET1702-74) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 µg/Lane) Lane 2: Ramos cell lysate (15 µg/Lane) Lane 3: PC-12 cell lysate (15 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 95 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of CD19 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.