Ctip2 Recombinant Rabbit Monoclonal Antibody [JF09-90]
cat.: ET1702-76
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JF09-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ctip2 aa 491-534 / 894. |
| Positive control: | LO2 cell lysate, THP-1 cell lysate, LO2, mouse brain tissue, human skin tissue, rat brain tissue, Jurkat. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IHC-Fr IF-Tissue |
1:1,000 1:50-1:200 1:200-1:1,000 1:50-1:100 1:500 1:200-1:500 |
| Uniprot #: | SwissProt: Q9C0K0 Human | Q99PV8 Mouse Entrez Gene: 314423 Rat |
| Alternative names: | ATL1 alpha ATL1 ATL1 beta ATL1 gamma ATL1-delta B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein B cell lymphoma/leukemia 11B B-cell CLL/lymphoma 11B (zinc finger protein) B-cell CLL/lymphoma 11B B-cell lymphoma/leukemia 11B BC11B_HUMAN BCL-11B Bcl11b BCL11B/TRDC fusion COUP TF interacting protein 2 COUP-TF interacting protein 2 COUP-TF-interacting protein 2 Ctip 2 CTIP-2 CTIP2 hRIT1 alpha hRit1 Radiation induced tumor suppressor gene 1 Radiation induced tumor suppressor gene 1 protein Radiation-induced tumor suppressor gene 1 protein Rit 1 Rit1 zinc finger protein hRit1 alpha ZNF856B |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-76, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Western blot analysis of Ctip2 on different lysates with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/500 dilution. Lane 1: LO2 cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-76) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of Ctip2 in LO2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-76) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Ctip2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-76, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-76) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of Ctip2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig8:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Ctip2 with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-76, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.