Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
Applications: | WB, IHC-P, IHC-Fr, IF-Tissue, IF-Cell |
Clonality: | Monoclonal |
Clone number: | JF09-90 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 96 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Ctip2 aa 491-534 / 894. |
Positive control: | LO2 cell lysate, THP-1 cell lysate, LO2, mouse brain tissue, human skin tissue, rat brain tissue, Jurkat. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue IF-Cell |
1:1,000 1:200-1:1,000 1:1,000-1:2,000 1:200-1:500 1:50-1:200 |
Uniprot #: | SwissProt: Q9C0K0 Human | Q99PV8 Mouse Entrez Gene: 314423 Rat |
Alternative names: | ATL1 alpha ATL1 ATL1 beta ATL1 gamma ATL1-delta B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein B cell lymphoma/leukemia 11B B-cell CLL/lymphoma 11B (zinc finger protein) B-cell CLL/lymphoma 11B B-cell lymphoma/leukemia 11B BC11B_HUMAN BCL-11B Bcl11b BCL11B/TRDC fusion COUP TF interacting protein 2 COUP-TF interacting protein 2 COUP-TF-interacting protein 2 Ctip 2 CTIP-2 CTIP2 hRIT1 alpha hRit1 Radiation induced tumor suppressor gene 1 Radiation induced tumor suppressor gene 1 protein Radiation-induced tumor suppressor gene 1 protein Rit 1 Rit1 zinc finger protein hRit1 alpha ZNF856B |
![]() |
Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P0) Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required |
![]() |
Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P7) Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required |
![]() |
Fig3:
Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:200 |
![]() |
Fig4:
Western blot analysis of Ctip2 on different lysates with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/500 dilution. Lane 1: LO2 cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-76) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
![]() |
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-76) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig6:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-76) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Ctip2 antibody (ET1702-76) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-76) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig8: ICC staining of Ctip2 in LO2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |