Neutrophil Elastase Recombinant Rabbit Monoclonal Antibody [JF098-6]
cat.: ET1702-78
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF098-6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Neutrophil Elastase aa 1-140 / 268. |
| Positive control: | HL-60 cell lysates, A549, Hela, HepG2, human bone marrow tissue, mouse bone tissue, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:100 1:50 1:50-1:200 |
| Uniprot #: | SwissProt: P08246 Human | Q3UP87 Mouse |
| Alternative names: | Bone marrow serine protease ELA2 ELANE Elastase 2 Elastase 2 neutrophil Elastase neutrophil expressed Elastase-2 ELNE_HUMAN GE Granulocyte derived elastase HLE HNE Human leukocyte elastase Leukocyte elastase Medullasin NE Neutrophil elastase PMN E PMN elastase Polymorphonuclear elastase SCN1 |
Images
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Fig1:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: HL-60 (Human acute promyelocytic leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: ET1702-78, 1/100, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig2: Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using anti-Neutrophil Elastase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse bone tissue using anti-Neutrophil Elastase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Neutrophil Elastase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Neutrophil Elastase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of Neutrophil Elastase on different lysates with Rabbit anti-Neutrophil Elastase antibody (ET1702-78) at 1/2,000 dilution. Lane 1: HL-60 (Human leukemia cell) cell lysate Lane 2: U-937 (Human histiocytic lymphom cell) cell lysate Lane 3: THP-1 (Human leukemia monocytic cell) cell lysate Lane 4: Jurkat (Human T lymphocytic leukemia cell) cell lysate (-) Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-78, 1/2000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti- Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 35 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.