ASPP2 Recombinant Rabbit Monoclonal Antibody [JF09-98]
cat.: ET1702-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF09-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 150 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human ASPP2. |
| Positive control: | MCF-7 cell lysate, human kidney tissue, human prostate tissue, mouse testis tissue. |
| Subcellular location: | Nucleus, perinuclear region. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:500-1:2,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: Q13625 Human | Q8CG79 Mouse |
| Alternative names: | 53BP2 Apoptosis stimulating of p53 protein 2 Apoptosis stimulating protein of p53 2 Apoptosis-stimulating of p53 protein 2 ASPP2 ASPP2_HUMAN BBP Bcl2 binding protein Bcl2-binding protein NY REN 51 antigen p53 binding protein 2 p53-binding protein 2 p53BP2 PPP1R13A Renal carcinoma antigen NY-REN-51 Tp53bp2 Tumor protein p53 binding protein 2 Tumor suppressor p53 binding protein 2 Tumor suppressor p53-binding protein 2 |
Images
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Fig1:
Western blot analysis of ASPP2 on different lysates with Rabbit anti-ASPP2 antibody (ET1702-79) at 1/500 dilution. Lane 1: MCF7 (Human breast cancer cell) Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-79, 1/500 in 5% NFDM/TBST,2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 150 kDa Observed band size: 150 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASPP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-79, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ASPP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-79, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASPP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-79, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.