S100P Recombinant Rabbit Monoclonal Antibody [JF95-08]
cat.: ET1702-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JF95-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human S100P aa 51-95 / 95. |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Hep G2, human spleen tissue, human stomach tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Cell projection. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000 1:500 1:200 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P25815 Human |
| Alternative names: | MIG9 Migration inducing gene 9 Protein S100-E Protein S100-P Protein S100P S100 calcium binding protein P S100 calcium-binding protein P S100 P S100E S100P S100P_HUMAN |
Images
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Fig1:
Western blot analysis of S100P on different lysates with Rabbit anti-S100P antibody (ET1702-82) at 1/1,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1702-82, 1/,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 10 kDa Observed band size: 10 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: Hep G2 (Human hepatocellular carcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: ET1702-82, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-S100P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-S100P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Application: Immunofluorescence (IF-tissue) Species: Human Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1702-82, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.