alpha 1 Antitrypsin Recombinant Rabbit Monoclonal Antibody [JF10-03]
cat.: ET1702-87
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JF10-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human alpha 1 Antitrypsin aa 1-418 / 418. |
| Positive control: | Human spleen tissue lysates, MCF-7, Hela, HepG2, human spleen tissue, human liver tissue, human breast carcinoma tissue. |
| Subcellular location: | Secreted, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01009 Human |
| Alternative names: | A1A A1AT A1AT_HUMAN AAT Alpha 1 antiproteinase Alpha 1 antitrypsin Alpha 1 antitrypsin null Alpha 1 protease inhibitor Alpha-1 protease inhibitor Alpha-1-antiproteinase alpha1 proteinase inhibitor Alpha1AT Dom1 PI PI1 PRO2275 Serine (or cysteine) proteinase inhibitor clade A member 1 Serine protease inhibitor 1-1 Serine protease inhibitor A1a Serpin A1 Serpin A1a Serpin peptidase inhibitor clade A member 1 Serpina1 Short peptide from AAT SPAAT Spi1-1 |
Images
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Fig1:
Western blot analysis of alpha 1 Antitrypsin on human spleen tissue lysates with Rabbit anti-alpha 1 Antitrypsin antibody (ET1702-87) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-87) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining alpha 1 Antitrypsin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining alpha 1 Antitrypsin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.