Myosin heavy chain Recombinant Rabbit Monoclonal Antibody [JF097-7]
cat.: ET1702-88
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: JF097-7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 224 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Myosin heavy chain aa 1220-1420 / 1939.
Positive control: Human skeletal muscle tissue lysates, rat skeletal muscle tissue, rat heart tissue, mouse smooth muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue lysates, human striated muscle tissue, human heart tissue, mouse heart tissue.
Subcellular location: Cytoplasm, myofibril.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:500-1:2,000
1:100-1:500
1:100
Uniprot #: SwissProt: P13533 Human | P12883 Human | Q02566 Mouse | P02563 Rat
Alternative names: cardiac muscle alpha isoform MYH6 MYH6_HUMAN MYH7 MYHC MyHC-alpha MyHC-beta MYHCA MYHCB Myosin heavy chain 6 Myosin heavy chain Myosin heavy chain cardiac muscle alpha isoform Myosin heavy chain cardiac muscle beta isoform Myosin-6
Images
ET1702-88_1.jpg Fig1: Western blot analysis of Myosin heavy chain on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1702-88_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Myosin heavy chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-88, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-88_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin heavy chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-88, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-88_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Myosin heavy chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-88, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-88_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myosin heavy chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-88, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-88_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin heavy chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-88, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1702-88_7.jpg Fig7: Western blot analysis of Myosin heavy chain on rat skeletal muscle tissue lysates with Rabbit anti-Myosin heavy chain antibody (ET1702-88) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 224 kDa
Observed band size: 224 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-88) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1702-88_8.jpg Fig8: Immunofluorescence analysis of paraffin-embedded rat heart tissue labeling Myosin heavy chain with Rabbit anti-Myosin heavy chain antibody (ET1702-88) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-88, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1702-88_9.jpg Fig9: Immunofluorescence analysis of paraffin-embedded human striated muscle tissue labeling Myosin heavy chain with Rabbit anti-Myosin heavy chain antibody (ET1702-88) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-88, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1702-88_10.jpg Fig10: Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Myosin heavy chain with Rabbit anti-Myosin heavy chain antibody (ET1702-88) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-88, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1702-88_11.jpg Fig11: Immunofluorescence analysis of paraffin-embedded mouse heart tissue labeling Myosin heavy chain with Rabbit anti-Myosin heavy chain antibody (ET1702-88) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-88, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1702-88_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Myosin heavy chain antibody (ET1702-88) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-88) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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