Thioredoxin Recombinant Rabbit Monoclonal Antibody [JF101-8]
cat.: ET1702-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JF101-8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Thioredoxin aa 78-105 / 105. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate, Mouse colon tissue lysate, Rat colon tissue lysate, rat big intestine tissue, human small intestine tissue, mouse kidney tissue, human kidney tissue, Hela. |
| Subcellular location: | Nucleus, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000-1:5,000 1:400-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P10599 Human | P10639 Mouse | P11232 Rat |
| Alternative names: | ADF ATL derived factor ATL-derived factor DKFZp686B1993 MGC61975 SASP Surface associated sulphydryl protein Surface-associated sulphydryl protein testicular tissue protein Li 199 THIO_HUMAN Thioredoxin thioredoxin delta 3 TRDX TRX 1 Trx TRX1 TXN TXN delta 3 TXN protein zgc:92903 |
Images
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Fig1:
Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse colon tissue lysate Lane 6: Rat colon tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat big intestine tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of Thioredoxin was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.