Thioredoxin Recombinant Rabbit Monoclonal Antibody [JF101-8]
cat.: ET1702-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JF101-8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Thioredoxin aa 78-105 / 105. |
| Positive control: | Hela cell lysate, 293T cell lysate, Hela, rat big intestine tissue, human small intestine tissue, mouse kidney tissue, human kidney tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:400-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P10599 Human | P10639 Mouse | P11232 Rat |
| Alternative names: | ADF ATL derived factor ATL-derived factor DKFZp686B1993 MGC61975 SASP Surface associated sulphydryl protein Surface-associated sulphydryl protein testicular tissue protein Li 199 THIO_HUMAN Thioredoxin thioredoxin delta 3 TRDX TRX 1 Trx TRX1 TXN TXN delta 3 TXN protein zgc:92903 |
Images
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Fig1:
Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of Thioredoxin was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat big intestine tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Thioredoxin antibody (ET1702-90) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.