Ras Recombinant Rabbit Monoclonal Antibody [JF10-11]
cat.: ET1702-94
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IF-Tissue, IP
Clonality: Monoclonal
Clone number: JF10-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Ras aa 21-64 / 189.
Positive control: MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse ovary tissue lysate, rat brain tissue lysate, zebrafish tissue lysate, Hela, MCF-7, PC-12.
Subcellular location: Cytoplasm, Cell membrane, Golgi apparatus, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IP
1:1,000-1:2,000
1:100-1:500
1:100-1:500
Use at an assay dependent concentration.
Uniprot #: SwissProt: P01111 Human | P01112 Human | P01116 Human | P08556 Mouse | P32883 Mouse | Q61411 Mouse | P08644 Rat | P20171 Rat | Q04970 Rat
Alternative names: C-BAS/HAS c-H-ras C-HA-RAS1 CTLO GTPase HRas GTPase KRas GTPase NRas H-Ras-1 H-RASIDX Ha-Ras HAMSV HRAS HRAS1 K RAS2A K RAS2B K RAS4A K RAS4B K-RAS KRAS KRAS1 KRAS2 N-RAS N-terminally processed NRAS NRAS1 p21ras RASH_HUMAN RASH1 RASK2 Transforming protein p21 v Ha ras Harvey rat sarcoma viral oncogene homolog v Ki ras2 Kirsten rat sarcoma viral oncogene homolog v ras neuroblastoma RAS viral oncogene homolog
Images
ET1702-94_1.jpg Fig1: Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (ET1702-94) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Mouse ovary tissue lysate (30 µg/Lane)
Lane 6: Rat brain tissue lysate (30 µg/Lane)

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1702-94_2.jpg Fig2: ICC staining of Ras in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-94_3.jpg Fig3: ICC staining of Ras in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-94_4.jpg Fig4: ICC staining of Ras in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.