PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71]
cat.: ET1702-96
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JF09-71
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 91/113 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PGC1 alpha aa 755-798 /798 and aa 269-323 /798.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate, SK-Br-3 cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q86YN6 Human | Q9UBK2 Human | 415302 Mouse | O70343 Mouse | Q8VHJ7 Mouse | Q811R2 Rat | Q9QYK2 Rat
Alternative names: LEM6 Ligand effect modulator 6 PERC Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha Peroxisome proliferative activated receptor, gamma, coactivator 1 Peroxisome proliferator activated receptor gamma coactivator 1 alpha peroxisome proliferator-activated receptor gamma coactivator 1 beta Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Peroxisome proliferator-activated receptor gamma coactivator 1-beta peroxisome proliferator-activated receptor gamma, coactivator 1 beta PGC 1 (alpha) PGC 1 alpha PGC 1v PGC-1(beta) PGC-1-alpha PGC-1-beta PGC-1-related estrogen receptor alpha coactivator PGC1 PGC1(alpha) PGC1A PGC1v PPAR gamma coactivator 1 alpha PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 PPAR gamma coactivator 1 PPAR gamma coactivator variant form PPAR gamma coactivator-1beta PPAR-gamma coactivator 1-alpha PPAR-gamma coactivator 1-beta PPARGC 1 alpha PPARGC-1-alpha PPARG......
Images
ET1702-96_1.jpg Fig1: Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/2,000 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C6 cell lysate
Lane 4: Human kidney tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Rat kidney tissue lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Rat brain tissue lysate
Lane 9: Mouse heart tissue lysate
Lane 10: Rat heart tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 91/113 kDa
Observed band size: 91/113 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.