Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JF09-71 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 91/113 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human PGC1 alpha aa 755-798 /798 and aa 269-323 /798. |
Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, SK-Br-3 cell lysate. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q86YN6 Human | Q9UBK2 Human | 415302 Mouse | O70343 Mouse | Q8VHJ7 Mouse | Q811R2 Rat | Q9QYK2 Rat |
Alternative names: | LEM6 Ligand effect modulator 6 PERC Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha Peroxisome proliferative activated receptor, gamma, coactivator 1 Peroxisome proliferator activated receptor gamma coactivator 1 alpha peroxisome proliferator-activated receptor gamma coactivator 1 beta Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Peroxisome proliferator-activated receptor gamma coactivator 1-beta peroxisome proliferator-activated receptor gamma, coactivator 1 beta PGC 1 (alpha) PGC 1 alpha PGC 1v PGC-1(beta) PGC-1-alpha PGC-1-beta PGC-1-related estrogen receptor alpha coactivator PGC1 PGC1(alpha) PGC1A PGC1v PPAR gamma coactivator 1 alpha PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 PPAR gamma coactivator 1 PPAR gamma coactivator variant form PPAR gamma coactivator-1beta PPAR-gamma coactivator 1-alpha PPAR-gamma coactivator 1-beta PPARGC 1 alpha PPARGC-1-alpha PPARG...... |
Fig1:
Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lane 4: Human kidney tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Mouse heart tissue lysate Lane 10: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 91/113 kDa Observed band size: 91/113 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |