PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71]
cat.: ET1702-96
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JF09-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91/113 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PGC1 alpha aa 755-798 /798 and aa 269-323 /798. |
| Positive control: | SK-Br-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Human kidney tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Mouse heart tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q86YN6 Human | Q9UBK2 Human | 415302 Mouse | O70343 Mouse | Q8VHJ7 Mouse | Q811R2 Rat | Q9QYK2 Rat |
| Alternative names: | LEM6 Ligand effect modulator 6 PERC Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha Peroxisome proliferative activated receptor, gamma, coactivator 1 Peroxisome proliferator activated receptor gamma coactivator 1 alpha peroxisome proliferator-activated receptor gamma coactivator 1 beta Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Peroxisome proliferator-activated receptor gamma coactivator 1-beta peroxisome proliferator-activated receptor gamma, coactivator 1 beta PGC 1 (alpha) PGC 1 alpha PGC 1v PGC-1(beta) PGC-1-alpha PGC-1-beta PGC-1-related estrogen receptor alpha coactivator PGC1 PGC1(alpha) PGC1A PGC1v PPAR gamma coactivator 1 alpha PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 PPAR gamma coactivator 1 PPAR gamma coactivator variant form PPAR gamma coactivator-1beta PPAR-gamma coactivator 1-alpha PPAR-gamma coactivator 1-beta PPARGC 1 alpha PPARGC-1-alpha PPARG...... |
Images
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Fig1:
Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lane 4: Human kidney tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Mouse heart tissue lysate Lane 10: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 91/113 kDa Observed band size: 91/113 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/1,000 dilution. Lane 1: His-tagged PGC1 alpha recombinant protein Lane 2: His-tagged PGC1 beta recombinant protein Lysates/proteins at 50 ng/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.