Anti-C3 antibody [JF10-30]
cat.: ET1702-99
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC/IF, FC
Clonality: Monoclonal
Clone number: JF10-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 100 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human C3 aa 1200-1240.
Positive control: HepG2 cell lysate, Hela cell lysate, NIH/3T3, HepG2.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  FC
  ICC/IF

1:500-1:1,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P01024 Human | P01027 Mouse
Alternative names: Acylation stimulating protein cleavage product antibody AHUS5 antibody ARMD9 antibody ASP antibody C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody C3 antibody CO3_HUMAN antibody Complement C3 antibody Complement C3c alpha' chain fragment 2 antibody Complement component 3 antibody Complement factor 3 antibody CPAMD1 antibody HEL S 62p antibody
Images
ET1702-99_1.jpg Fig1: Western blot analysis of C3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
ET1702-99_2.jpg Fig2: ICC staining of C3 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-99_3.jpg Fig3: Flow cytometric analysis of C3 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-99, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.