SERCA2 Recombinant Rabbit Monoclonal Antibody [JM10-20]
cat.: ET1703-01
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JM10-20
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 115 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SERCA2 aa 999-1,042 / 1,042.
Positive control: A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, A549, Hela, MCF-7, human tonsil tissue, human kidney tissue, mouse skeletal muscle tissue, mouse skin tissue, human liver carcinoma tissue, mouse kidney tissue, mouse heart tissue.
Subcellular location: Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P16615 Human | O55143 Mouse | P11507 Rat
Alternative names: AT2A2_HUMAN Atp2a2 ATP2B ATPase Ca++ transporting cardiac muscle slow twitch 2 Calcium pump 2 Calcium-transporting ATPase sarcoplasmic reticulum type Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform Cardiac Ca2+ ATPase DAR DD Endoplasmic reticulum class 1/2 Ca(2+) ATPase MGC45367 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 SERCA 2 SERCA2 serca2a slow twitch skeletal muscle isoform SR Ca(2+)-ATPase 2
Images
ET1703-01_1.jpg Fig1: Western blot analysis of SERCA2 on different lysates with Rabbit anti-SERCA2 antibody (ET1703-01) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: A549 cell lysate (no heat)
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 cell lysate (no heat)
Lane 5: PC-12 cell lysate
Lane 6: PC-12 cell lysate (no heat)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 115 kDa
Observed band size: 115 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-01) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1703-01_2.jpg Fig2: ICC staining of SERCA2 in A549 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-01, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-01_3.jpg Fig3: ICC staining of SERCA2 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-01, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-01_4.jpg Fig4: ICC staining of SERCA2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-01, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-01_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SERCA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-01_12.jpg Fig12: Flow cytometric analysis of SERCA2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-01, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.