beta 2 Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody [JM102-06]
cat.: ET1703-04
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JM102-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ADRB2 aa 369-400 / 413. |
| Positive control: | A431 cell lysate, human liver tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, zebrafish tissue lysates, mouse liver tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane, Early endosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000-1:5,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P07550 Human | P18762 Mouse | P10608 Rat |
| Alternative names: | ADRB2 ADRB2_HUMAN ADRB2R ADRBR Adrenergic beta 2 receptor surface Adrenoceptor beta 2 surface B2AR BAR beta 2 adrenoceptor Beta 2 adrenoreceptor Beta-2 adrenergic receptor Beta-2 adrenoceptor Beta-2 adrenoreceptor BETA2AR Catecholamine receptor OTTHUMP00000160386 |
Images
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Fig1:
Western blot analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody (ET1703-04) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Human liver tissue lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 55-100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of beta 2 Adrenergic Receptor on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-beta 2 Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta 2 Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.